Dual monochromators provide the ability for. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. SpectraMax M5 microplate reader. The patented AutoPMT optimization of the SpectraMax M5 reader adjusts the fluorescence detector to each sample well's concentration and normalizes the raw data, extending the. 7°C and the bottom to 36. Figure 1. In either case. ItSpectraMax® M5多功能读板机为使用者提供了除化学发光以外的其他检测方法，例如：光吸收，荧光强度，荧光偏振和时间分辨荧光。 SpectraMax® L和SpectraMax® M5酶标仪均用SoftMax® Pro软件进行操控和设置，该软件含有CellTiter-Glo检测模板和其他120多个分析模板，这些. Keywords: spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software,. No accessories are needed and SoftMax® Pro. the IMAP Platform and smoothly transition into high-throughput screening. The IP-One assay can be used to characterize compounds that act on Gq-coupled receptors in either adherent or suspension cells. 0200-0729. spr * SpectraMax i3(x) SpectraTest ABS2 v1. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. For more information please visit us at: Devices SpectraMax M5 Multi-Mode Microplate Reader Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. 检测模式包括： • 紫外-可见光 光吸收 (Abs) • 荧光强度 (FI) • 化学发光 (Lum) • 时间分辨荧光 (TRF) • 荧光偏振 (FP) SpectraMax M5e 在 M5 基础上通过了 Cisbio HTRF 检测认证而增加了应用范围。. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Devices SpectraMax i3, SpectraMax Paradigm, or SpectraMax M5 Multi-Mode Microplate Readers. deliver increased capabilities over the M2/M2e Dual-Mode Readers at a more affordable price than the top-of-the-line M5/M5e Multi-Mode Readers, MDS Analytical Technologies can now meet an. Plates were mixed for 2 min on an orbital shaker and then incubated at room temperature for 10 min. A new microplate luminometry for the toxicity bioassay of chemicals on firefly luciferase, was developed using the multifunctional microplate reader (SpectraMax M5) to measure the luminous intensity of luciferase. IMAP Assay Kits IMAP Assay Kits provide a turnkey solution to evaluate . . 1. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. The. 4. com A. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes. 4. Neutrophils from six HC patients were stimulated by PMA with or without pretreatment of SIRL-1 mAb, and then co-cultured with autologous monocytes for 12 h. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Multimode Reader Comparison Chart. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. Samples were measured together with a corresponding reference solvent contained in a. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. How do I do this? Note: Check the Applies to section at the bottom of the page to make this is the. SOFTmax PRO UserÕs Manual iii Contents Figures . Description. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes 6. 5. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. DOCUMENTATION. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date: 5/20/2019 9:24:38 AM 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide Place the plate into a SpectraMax M5 e plate reader (Molecular Devices). The following document is intended to demonstrate setup of this. 1. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. Choose from a three- (M3), four- (M4), or Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The excitation and the emission wavelengths on. D 1 1. 0200-7027. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. D 1. FlexStation® 3 Multi-Mode Microplate Reader. 1. Cavicide (Warehouse, Cat # 79300360) 6. The chamber contains springs that position the cuvette in the proper alignment for a read. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. SpectraMax M2 and SpectraMax M2 e are controlled by an external computer running SoftMax Pro software which provides integrat ed instrument control, data display, andSpectraMax M5, Analyst HT or Analyst GT. Recertification is recommended every two years. Step 4. HTRF is a proprietary time-resolved fluorescence technology that overcomes SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. Statistical analyses The statistical analyses were performed using Graph Prism version 7. The Gemini XPS Reader has dual monochromators that allow users to utilize new and novel dyes without purchasing expensive filter sets. Table of Contents. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid-The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. When used with SoftMax® Pro Data Acquisition and Analysis Software, it can automatically normalize the well absorbance to a cuvette equivalentAnalytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). 5. View the Molecular Devices websiteSpectraMax® M5 multimode microplate reader from Molecular Devices to measure both reporters in a completely homogeneous manner, in the same well of a 96-well plate, without cell lysis. Molecular Devices also offers a mass spectrometry-based solution for food safety testingMOLECULAR DEVICES SpectraMax M5 Microplate Reader For Sale. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Optimized for fluorescence microplate readers. Cet appareil ISM est confomre à la norme NMB-001 du Canada. 1) SpectraMax Glo Steady Luc Reporter Assay (6. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. 00 USD. 4. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. 00 USD. For more sample throughput, the system canMolecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. . 72 MB. viiProducts : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, SpectraMax M4 reader, SpectraMax M3 reader What is the best way to use fluorescence (FL) mode with cuvettes? 1. Features. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. ) Click the "OK" button on SoftMax Pro Setup dialog box . Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Open the Softmax Pro software and select the FP option. Currently we have over 300 Windows 7/10 compatible instrument drivers to control equipment from 147 manufacturers, meaning that we are likely to have your instrument already in our library. The resulting OD 426 values are visually represented above as a color map generated in the plate section of SoftMax ® Pro Software. A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. acquired on a SpectraMax M5 (Molecular Devices). ” SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. OptimizingAbsorbanceReadMode The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. 2. These all-inclusive assay kits are ready to use in assay validation or screening. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. Other technologies such as imaging. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. . type Clear. The corrected absorbance is within 5% of the. 01) between the GFP-transfected and mock-transfected cells at an initial seeding. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). 9000-0756. 72 MB. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5e• SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. e. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. The chamber. 314 low lamp level warning. Download PDF versions of the SpectraMax M5 User Guides from this page. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. fluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiter Created DateThe Spectramax M5 has similar detection capability to the Analyst GT and LMax II 384 (Figure 4), so we are confident that its performance is also similar when measuring the enzymes in cell lysates. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. 0 out of 5 $ 17,999 $ 16,995. . There were 12 replicate wells per dilution in both 96-well and 384- well plates. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. . The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. The following document is intended to demonstrate setup of this. Flexible for 6-well to 384-well microplates. The SpectraMax M5 is a microplate reader that can be used for various applications, such as ELISA, Western blot, or cell culture. 5. 2. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Terbium-based TR-FRET assays. . In other words, I carry out equilibrium folding/unfolding titrations of proteins and monitor the process using. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. The chamber contains. SpectraMax Quant dsDNA Assay Kits. Exhibit with us {{>currencyLabel}} Back. 2. 5. Our absorbance plate readers feature our PathCheck Sensor. Fix: While proper use of the Shake button is to hold the button down for the duration of the desired mixing time, repeated sequential clicking is now allowed. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 2 SpectraMax MiniMax300ImagingCytometer(cytometer) 3. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Plate chamber design of the SpectraMax category 1 microplate readers. Fluorescence spectra and UV–vis spectra were recorded on a SpectraMax ® M5 multi-mode microplate reader (Molecular Devices). Molecular Devices SpectraMax 190 Microplate Absorbance Spectrophotometer. type Clear. For very concentrated samples, use a minimum2. EarlyTox Live Cell Assay (6. Bookings are free, but you will need to use iLab to book. Read fluorescence polarization at room temperature (RT, 19–25°C). 3. SpectraMax M5, Analyst HT or Analyst GT. Efects of luciferase concentration, luciferin concentration, ATP concentration, pH, temperature, and reaction time on the. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. . LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Measure assay absorbance values quickly with minimal background noise on the SpectraMax iD5 reader. This application note describes how to. it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. Table of Contents. Call 516-939-0888 to check current. DNA provides the same sensitivity as a spectrophotometer. 314 low lamp level warning. Wavelength Range. Also refer to this Multimode Reader Comparison Chart. Molecular Devices SpectraMax M5e User Manual (86 pages) Multi-Mode Microplate Readers. . used. 1. . Choose from a three- (M3), four- (M4), orAll general chemicals and solvents were commercially available and used as received. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. SpectraMax M5 and the Analyst GT. Detection of pro-inflammatory factors. 0 (IBM Corporation, Armonk, NY, USA). Third this recommendation. Wavelength Range. EUROPE. The SpectraMax M5/M5e & FlexStation 3 instruments use SoftMaxPro® Data Acquisition & Analysis Software. . Choose from a three- (M3), four- (M4), orSpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). 95. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The M5 has two PMTs. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. 6. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. Instructions on the proper use of the SpectraMax M5 Plate Reader. This monochromator-based instrument allows users to select optimal wavelengths for any chosen fluorophore without the need to purchase additional filter sets. SoftMax Pro Software combines reader control and data analysis and provides sophisticated curve fitting and statistics for more in-depth bioassay analysis. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4 SpectraMax® M5 Multi-Mode Microplate Reader. . LMax, SpectraMax ®, VersaMax™, VMax , EMax , UVMax, FlexStation®, and ThermoMax benchtop Microplate Readers. Figure 1. Last lab had ours for years and never had a problem with it. com A. This analysis indicated that, after 48 hours incubation, there was a significant difference (P < 0. ® reader to the computer. Refurbished. D 1 1. When the user applies a set temperature of 37°C, the top is heated to 37. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15. BRET does not work on the Spectramax M5 due to a sensitivity issue and to the fact that the BRET assay has very weak signal in general. Settings not required by a reader are indicated by ‘---’. $2,264. Table of Contents. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. 40266 Email: drugdiscoverytech@lifetech. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. 315 can't find zero order. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. 190-1000 nm, tunable in 1 nm increments. SpectraMax M5 Multi-Mode Microplate Reader. Offer Ends In: 18 Days 19: 38: 21. . . 7°C (gradient heating design). 190-1000. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Description 1. Set the excitation at 531 nm and emission at 595 nm. My experiments primarily involve monitoring the change in tryptophan fluorescence with time, using 96. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. If you do not have an iLab account you must first make one: Calcium Assay Kits are the platform of choice for measuring changes of intracellular calcium for drug discovery and basic research. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Two sequential measurements should be carried out: at 620 nm for the cryptate emission, and at 665 nm for the specific signal emitted by the acceptor. We would like to show you a description here but the site won’t allow us. spr SpectraMax iD5 SpectraTest ABS2 v1. The M5 can read volumes down to 2uL with the uMax Low. Mass spectra were recorded using an HPLC-System Series 1100 coupled with ESI-single. For wavelengths in the UV range above 220 nm,. If you do not have an iLab account you must first make one: SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability and also uses patented PathCheck® Pathlength Measurement Technology – the only temperature independent pathlength correction for microplates. free shipping. The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Examples: “Press carefully to engage,” “ Do not press down. In this product video, see how the SpectraMax M5 quickly reads samples. together with SpectraMax, VersaMax, VMax, and EMax microplate readers from Molecular Devices, offer a quality assurance screening solution for the detection of melamine contamination in such food products as milk, infant formula, pet food, and confectionery. . Softmax Pro GxP Software 6. 発光モードに対応した機種（SpectraMax M5e/M5/M4/M3）では、発光モード専用のフォトンカウント検出器も搭載しました。 蛍光に特化した検出器を流用するのではなく、専用の光学系を採用することで、発光、TR. 0 mm wide, and heights from 14 mm to 15 mm. . Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. The drawing shows a standard microplate of 15 mm height covered with a lid. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. The SpectraMax M5 is a microplate reader that can be used for various applications,. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. 00 USD. Unit comes with software and computer. Molecular Devices has collaborated with various assay partners to optimize and validate homogeneous and heterogeneous biochemical- or cell-based assay performance on the SpectraMax Microplate Reader platform. book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax® M5 Multi-Mode Microplate Reader is capable of measuring UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, and TRF. Monday to Friday 7am - 5pm Pacific Time. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Optimal instrument settings are the same for all three readers. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. . SpectraMax Injector Cartridge with SmartInject™ Technology. Molecular Devices Spectramax M5 luminometer: Krishnamurthy lab-Praveen (lab WO3213, x4605) Spectramax plate reader: yes Beckman LS6000IC Scintillation counter: Bill Taylor (office WO4262B, x1966) WO3242 liquid scintillation counter Department backup -80oC freezer: Song-Tao Liu (WO4254, x7857) Facilities Facility: Department. Click SoftMax Pro Software User Guide. 9000-0756. 000). Introduction. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 26 5039228D Note. IMAP Assay Kits IMAP Assay Kits provide a turnkey solution to evaluate . Fluorescence Microplate Reader Comparison Chart. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. . Streamline BCA-based protein quantitation on the SpectraMax iD5 reader. 3. Molecular Devices ®(MD) SpectraMax 340PC reader MD VersaMax™ Tunable Microplate readers MD SpectraMax® L Microplate Luminometers MD SpectraMax® M5 systems MD ®SpectraMax 96/384-well plate reader Gemini fluorescence plate reader Automation Hamilton Microlab® STAR™ liquid handling systemThe SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Choose cables that meet the requirement described in this appendix. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). Some luminescence applications, such as gene reporter assays, may require a luminometer with photon counting detection for greater sensitivity, such as the SpectraMax M5, Analyst GT or Analyst HT, or LMax II. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. Softmax Pro GxP Software 6. SpectraMax M5e Multi-Mode Microplate Reader. In addition, the SpectraMaxMolecular Devices. . 005) Photometric PrecisionThe Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Maximum signal with lowest background. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. SpectraMax M series, Gemini, and SpectraMax iDx readers have xenon flash lamps,. Add to Cart. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Published Dec 16, 2020 | Updated Dec 16, 2020. 4. . Data collected by Analyst® and FLIPR® Systems can also be imported and analyzed in the software. Optical absorbance was measured at 570 nm on a SpectraMax M5 microplate reader. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. 1) EarlyTox Glutathione Assay (6. 3: The cuvette chamber. Absorbance measurements of ADMA were acquired using a Biotek Cytation 5-plate reader. It does not work as expected. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesand emission wavelengths for SpectraMax M5 fluorescence microplate readers are as follows: excitation at 490 nm and emission at 525 nm, with 515-nm emission cutoff filter (note that these are different from the wavelengths recommended for filter-based readers in the Quant-iT PicoGreen product insert). SpectraMax fluorescence microplate reader with SoftMax® Pro Software, is a quick, sensitive detection method for proteins. 7" color, capacitive touch screen. Lower limits of detection for ATP determined using the ATPlite . Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. Customer Satisfaction is our number one goal All lab equipment in stock. SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. 0 out of 5 $ 17,999 $ 16,995. SpectraMax M5 is a fantastic plate reader - easily my #2 behind PerkinElmer (if you can afford them). Step 3. 304 signal level saturation. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Cavicide (Warehouse, Cat # 79300360) 6. Table of Contents. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Methods Preparation of reagents and standards. SPECTRAmax GEMINI-XS spectrofluorometer was able to detect a specific GFP signal at all cell numbers as determined by One Way Analysis of Variance (ANOVA) (Table 1). 1 or greater versions. For example, microplates containing different samples can be read using the same orOn SpectraMax L, 1 RLU = 1 count per second for photon counting mode only. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Category 2 and 3 microplate readers use gradient heating as well. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Detection modalities are shown in Table 1-1. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Cavicide (Warehouse, Cat # 79300360) 6. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. HTRF is a proprietary time-resolved fluorescence technology that overcomesSpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Both full length human PI4KIIIB and the truncated PI4KIIIB crystal construct with the c-term were run at 200 nM. When the correct COM port is selected, the. 3. The SpectraMax® Gemini™ XPS Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. Absorbance (UV – VIS) Fluorescence; Glow luminescence; Time-resolved fluorescence; Fluorescent polarization; ABI Viia7 qPCR instrument (96 format) – SYBR/FAM/VIC/ROX calibrated. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. Typical LLDs were approximately 1–2 fg/well in the Analyst GT and 2–3 fg/well in the SpectraMax M5. Note: for the THUNDER assay, performance of a SpectraMax M5e reader will be equivalent to that of a SpectraMax M5 reader. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. There were 12 replicate wells per dilution in both 96-well and 384- well plates. The dual monochromator optics allow the widest range of applications to be utilized for bior. For example, microplates containing different samples can be read using theThe interval between reads was set at 15 s or 60 s, respectively. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. The excitation and the emission. Find many great new & used options and get the best deals for Molecular Devices SpectraMax M5 Microplate Reader with 90-Day Warranty at the best online prices at eBay! Free shipping for many products!Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory.